![]() ![]() Image analysis was performed with Aperio Positive Pixel Count algorithm to quantify the proportion of C4d-positive pixels in the area analysed. Two layers of annotations were created: layer of glomeruli and the remaining non-glomerular area. The same slides were scanned by Aperio ScanScope scanner. We aimed to explore the potential of digital pathology tools to obtain quantitative and reproducible measure of C4d deposition in the renal allograft tissue.ģ4 routine kidney allograft biopsies immunohistochemically stained for C4d were included in the study and were evaluated by a qualified pathologist twice, recording an approximate percentage of positive PTC and glomerular area. In the Banff 07 classification of renal allograft pathology, semi-quantitative evaluation of the proportion of C4d-positive peritubular capilaries (PTC) is used. Complement C4d component deposition in kidney allograft biopsies is an established marker of antibody-mediated rejection.
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